Membrane pore architecture of a cytolytic toxin from Bacillus thuringiensis
نویسندگان
چکیده
منابع مشابه
Membrane pore architecture of a cytolytic toxin from Bacillus thuringiensis.
To investigate the membrane pore structure of Cyt2Aa1 toxin from Bacillus thuringiensis, 14 single-cysteine substitutions of the toxin were constructed. Five of these mutants (L172C, V186C, L189C, E214C and L220C) yielded characteristic products when processed by proteinase K; other mutants were degraded by this enzyme. Mutants that yielded characteristic proteolysed products and wild-type toxi...
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Using the shuttle vector pBU4, the mosquitocidal toxin gene mtx1 from Bacillus sphaericus strain SSII-1 was introduced into an acrystalliferous strain of B. thuringiensis both individually and in combination with the accessory protein gene p20 and the cytolytic protein gene cyt1Aa from B. thuringiensis subsp. israelensis. Bioassay results indicated that the recombinants B-pMT4(Mtx1) and B-pMT9(...
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The Bacillus thuringiensis var. israelensis (Bti) cytolytic toxin is hypothesized to exert its toxic activity via pore formation in the cell membrane as a result of the aggregation of several monomers. To gain insight into the toxin's mode of action, 2 putative hydrophobic 22 amino acid peptides were synthesized and characterized spectroscopically and functionally. One peptide corresponded to t...
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To investigate whether membrane proteases are involved in the activity of Bacillus thuringiensis insecticidal toxins, the rate of pore formation by trypsin-activated Cry1Aa was monitored in the presence of a variety of protease inhibitors with Manduca sexta midgut brush border membrane vesicles and by a light-scattering assay. Most of the inhibitors tested had no effect on the pore-forming abil...
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Bacillus thuringiensis (Bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. The widespread use of Bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. Downstream separation/purification efficacy governs the marketabi...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2000
ISSN: 0264-6021
DOI: 10.1042/0264-6021:3500275